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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
05/12/2016 |
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Actualizado : |
07/12/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CARLSON, J. S.; GIANNITTI, F.; VALKIÜNAS, G.; TELL, L. A.; SNIPES, J.; WRIGHT, S.; CORNEL, A. J. |
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Afiliación : |
JENNY S. CARLSON, Mosquito Control Research Laboratory, Department of Entomology and Nematology, Kearney Agriculture Center, University of California; FEDERICO GIANNITTI, University of Minnesota, Saint Paul, MN, USA; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; University of California, Davis, CA, USA; GEDIMINAS VALKIÜNAS, Nature Research Centre, Akademijos 2; LISA A. TELL, Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California; JOY SNIPES, Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California; STAN WRIGHT, Sacramento-Yolo Mosquito Vector and Control District; ANTHONY J. CORNEL, Mosquito Control Research Laboratory, Department of Entomology and Nematology, Kearney Agriculture Center, University of California; Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, University of California. |
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Título : |
A method to preserve low parasitaemia Plasmodium-infected avian blood for host and vector infectivity assays. |
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Complemento del título : |
Methodology. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Malaria Journal, 2016, v.15 (5): Article number 1198. OPEN ACCESS |
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ISSN : |
1475-2875 |
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DOI : |
10.1186/s12936-016-1198-5 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 08 July 2015 // Accepted 01 March 2016 //First Online 11 March 2016. |
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Contenido : |
ABSTRACT.
Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated intravenously into canaries and once infection was established, Culex stigmatosoma, Cx. pipiens and Cx. quinquefasciatus mosquitoes were then allowed to feed on the infected canaries to validate the efficacy of this method for mosquito vector competence assays. Results: Storage of Plasmodium spp. - infected donor blood at 4°C yielded viable parasites for 48 h. All five experimentally-infected canaries developed clinical signs and were infectious. Pathologic examination of three canaries that later died revealed splenic lesions typical of avian malaria infection. Mosquito infectivity assays demonstrated that Cx. stigmatosoma and Cx. pipiens were competent vectors for Plasmodium cathemerium. Conclusions: A simple method of collecting and preserving avian whole blood with malaria parasites of low parasitaemia (?0.0005 %) was developed that remained viable for further experimental bird and mosquito infectivity assays. This method allows researchers interested in conducting infectivity assays on target Plasmodium spp. to collect these parasites directly from nature with minimal impact on wild birds.
© 2016 Carlson et al. MenosABSTRACT.
Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated int... Presentar Todo |
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Palabras claves : |
ANIMAL EXPERIMENT; AVIAN MALARIA; BIRD INOCULATION; BLOOD PRESERVATION; CULEX spp. VECTORS; EXPERIMENTAL INFECTION; PATHOLOGY; PLASMODIUM CATHEMERIUM. |
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Thesagro : |
MODELOS ANIMALES. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12151/1/s12936-016-1198-5.pdf
https://malariajournal.biomedcentral.com/articles/10.1186/s12936-016-1198-5
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Marc : |
LEADER 03718naa a2200337 a 4500
001 1056201
005 2018-12-07
008 2016 bl uuuu u00u1 u #d
022 $a1475-2875
024 7 $a10.1186/s12936-016-1198-5$2DOI
100 1 $aCARLSON, J. S.
245 $aA method to preserve low parasitaemia Plasmodium-infected avian blood for host and vector infectivity assays.$h[electronic resource]
260 $c2016
500 $aArticle history: Received 08 July 2015 // Accepted 01 March 2016 //First Online 11 March 2016.
520 $aABSTRACT. Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated intravenously into canaries and once infection was established, Culex stigmatosoma, Cx. pipiens and Cx. quinquefasciatus mosquitoes were then allowed to feed on the infected canaries to validate the efficacy of this method for mosquito vector competence assays. Results: Storage of Plasmodium spp. - infected donor blood at 4°C yielded viable parasites for 48 h. All five experimentally-infected canaries developed clinical signs and were infectious. Pathologic examination of three canaries that later died revealed splenic lesions typical of avian malaria infection. Mosquito infectivity assays demonstrated that Cx. stigmatosoma and Cx. pipiens were competent vectors for Plasmodium cathemerium. Conclusions: A simple method of collecting and preserving avian whole blood with malaria parasites of low parasitaemia (?0.0005 %) was developed that remained viable for further experimental bird and mosquito infectivity assays. This method allows researchers interested in conducting infectivity assays on target Plasmodium spp. to collect these parasites directly from nature with minimal impact on wild birds. © 2016 Carlson et al.
650 $aMODELOS ANIMALES
653 $aANIMAL EXPERIMENT
653 $aAVIAN MALARIA
653 $aBIRD INOCULATION
653 $aBLOOD PRESERVATION
653 $aCULEX spp. VECTORS
653 $aEXPERIMENTAL INFECTION
653 $aPATHOLOGY
653 $aPLASMODIUM CATHEMERIUM
700 1 $aGIANNITTI, F.
700 1 $aVALKIÜNAS, G.
700 1 $aTELL, L. A.
700 1 $aSNIPES, J.
700 1 $aWRIGHT, S.
700 1 $aCORNEL, A. J.
773 $tMalaria Journal, 2016$gv.15 (5): Article number 1198. OPEN ACCESS
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
06/06/2018 |
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Tipo de producción científica : |
Documentos |
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Autor : |
BELDARRAIN, G.; ÁVILA, S. |
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Afiliación : |
GISELA BELDARRAIN MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIFLOR STELLA ÁVILA SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Estudio de las poblaciones de Sclerotium orzyzae en el suelo y su relación con la podredumbre del tallo del arroz. |
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Complemento del título : |
Manejo de enfermedades. II. Estudio de las poblaciones de hongos causantes de enfermedades del tallo. |
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Fecha de publicación : |
2004 |
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Fuente / Imprenta : |
ln: INIA TREINTA Y TRES. Arroz: resultados experimentales 2003-2004. Treinta y Tres (Uruguay): INIA, 2004. |
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Páginas : |
cap. 4, p. 17-22. |
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Serie : |
(INIA Serie Actividades de Difusión; 373) |
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Idioma : |
Español |
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Thesagro : |
ARROZ; ENFERMEDADES DE LAS PLANTAS; FUNGICIDAS; SCLEROTIUM ORYZAE. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10054/1/Ad-373-cap.4-p.17-22.pdf
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Marc : |
LEADER 00677naa a2200193 a 4500
001 1030876
005 2018-06-06
008 2004 bl uuuu u00u1 u #d
100 1 $aBELDARRAIN, G.
245 $aEstudio de las poblaciones de Sclerotium orzyzae en el suelo y su relación con la podredumbre del tallo del arroz.
260 $c2004
300 $acap. 4, p. 17-22.
490 $a(INIA Serie Actividades de Difusión; 373)
650 $aARROZ
650 $aENFERMEDADES DE LAS PLANTAS
650 $aFUNGICIDAS
650 $aSCLEROTIUM ORYZAE
700 1 $aÁVILA, S.
773 $tln: INIA TREINTA Y TRES. Arroz: resultados experimentales 2003-2004. Treinta y Tres (Uruguay): INIA, 2004.
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